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Molecular and Cellular Biosciences at Wake Forest University


Wake Forest University Graduate School » Molecular and Cellular Biosciences

Derek Parsonage, Ph.D.

Derek Parsonage, Ph.D.
Education & Training
  BSc University Birmingham-United Kingdom 1980
  PhD University Birmingham-United Kingdom 1984
Fellowship
  University of Rochester Sch of Med & Dentistry 1988
Memberships
American Society of Emergency Radiology

 

Nelson KJ, Parsonage D, Karplus PA, Poole LB. Evaluating peroxiredoxin sensitivity toward inactivation by peroxide substrates. Methods Enzymol. 2013;527():21-40.

 

Keyes JD, Nelson K, Parsonage D, Daniel L, Furdui C, Poole L. Modulation of signaling proteins by reversible cysteine modification [abstract]. FASEB J. 2013;27():993.3.

 

 

Debnath A, Parsonage D, Andrade RM, He C, Cobo ER, Hirata K, Chen S, Garcia-Rivera G, Orozco E, Martinez MB, Gunatilleke SS, Barrios AM, Arkin MR, Poole LB, McKerrow JH, Reed SL. A high-throughput drug screen for Entamoeba histolytica identifies a new lead and target. Nat Med. 2012;18(6):956-960.

 

Nelson KJ, Parsonage D, Van Swearingen AED, Yuan Y, Salsbury FR, Hall A, Karplus PA, Poole LB. Specific residues in peroxiredoxins promote peroxide reactivity through effects on cysteine pKa, transition state stabilization and oligomerization [abstract]. Free Radic Biol Med. 2012;53(Suppl 2):S151.

 

Poole L, Karplus PA, Nelson K, Parsonage D. Active site and interface communication regulating peroxiredoxin functions [abstract]. Free Radic Biol Med. 2012;53(Suppl 2):S8.

 

 

 

Reeves SA, Parsonage D, Nelson KJ, Poole LB. Kinetic and thermodynamic features reveal that Escherichia coli BCP is an unusually versatile peroxiredoxin. Biochemistry. 2011;50(41):8970-8981.

 

Parsonage D, Desrosiers DC, Hazlett KRO, Sun Y, Nelson KJ, Cox DL, Radolf JD, Poole LB. Broad specificity AhpC-like peroxiredoxin and its thioredoxin reductant in the sparse antioxidant defense system of Treponema pallidum. Proc Natl Acad Sci U S A. 2010;107(14):6240-6245.

 

Parsonage D, Reeves SA, Karplus PA, Poole LB. Engineering of fluorescent reporters into redox domains to monitor electron transfers. Methods Enzymol. 2010;474():1-21.

 

Hall A, Parsonage D, Poole LB, Karplus PA. Structural evidence that peroxiredoxin catalytic power is based on transition-state stabilization. J Mol Biol. 2010;402(1):194-209.

 

Parsonage D, Newton GL, Holder RC, Wallace BD, Paige C, Hamilton CJ, Dos Santos PC, Redinbo MR, Reid SD, Claiborne A. Characterization of the N-acetyl-alpha-D-glucosaminyl l-malate synthase and deacetylase functions for bacillithiol biosynthesis in Bacillus anthracis. Biochemistry. 2010;49(38):8398-8414.

 

Coffman LG, Parsonage D, D'Agostino RD Jr, Torti FM, Torti SV. Regulatory effects of ferritin on angiogenesis. Proc Natl Acad Sci U S A. 2009;106(2):570-575.

 

Hall A, Parsonage D, Horita D, Karplus PA, Poole LB, Barbar E. Redox-dependent dynamics of a dual thioredoxin fold protein: evolution of specialized folds. Biochemistry. 2009;48(25):5984-5993.

 

Wallen JR, Mallett TC, Boles W, Parsonage D, Furdui CM, Karplus PA, Claiborne A.Crystal structure and catalytic properties of Bacillus anthracis CoADR-RHD: implications for flavin-linked sulfur trafficking. Biochemistry. 2009;48(40):9650-9667.

 

Colussi T, Parsonage D, Boles W, Matsouka T, Mallett TC, Karplus PA, Claiborne A.Structure of alpha-glycerophosphate oxidase from Streptococcus sp.: a template for the mitochondrial alpha-glycerophosphate dehydrogenase. Biochemistry. 2008;47(3):965-977.

 

Parsonage D, Karplus PA, Poole LB. Substrate specificity and redox potential of AhpC, a bacterial peroxiredoxin. Proc Natl Acad Sci U S A. 2008;105(24):8209-8214.

 

Nelson KJ, Parsonage D, Hall A, Karplus A, Poole LB. Cysteine pKa values for the bacterial peroxiredoxin AhpC. Biochemistry. 2008;47(48):12860-68.

 

Nicely NI, Parsonage D, Paige C, Newton GL, Fahey RC, Leonardi R, Jackowski S, Mallett TC, Claiborne A. Structure of the type III pantothenate kinase from Bacillus anthracis at 2.0 angstrom resolution: implications for coenzyme A-dependent redox biology. Biochemistry. 2007;46(11):3234-3245.

 

Parsonage D, Nelson KJ, Day AE, Poole LB. Substrate specificity and chemical characterization of a bacterial peroxiredoxin, AhpC [abstract]. Free Radic Biol Med. 2007;43(Suppl 1):S113.

 

My research focuses on several enzymes expressed by the Gram positive bacteria Enterococci. These enzymes include the flavoproteins NADH peroxidase, NADH oxidase and -glycerophosphate oxidase. These have all been cloned, sequenced and overexpressed in E. coli. The enzymic mechanisms of these three enzymes are being studied by a combination of traditional spectrophotometric measurements and the creation of site-directed mutants to dissect the enzymatic reactions. The genetic regulation of glycerol metabolism in Enterococcus is also being studied. This involves the investigation of transcriptional control of the glp operon, which has been cloned. This study includes measuring transcription (mRNA) levels and the use of reporter genes fused to the glp operon. promoter. Another way in which glycerol metabolism is controlled is by phosphorylation of glycerol kinase, which activates the kinase and hence controls the flux through the catabolic pathway. The gene for enterococcal glycerol kinase has been closed and overexpressed in E. coli. This protein is now being used to study the control of enzyme activity within the cell.

Derek Parsonage - Glycerol Uptake