Daniel B Kim-Shapiro, Ph.D.

Molecular and Cellular Biosciences at Wake Forest University


Wake Forest University Graduate School » Molecular and Cellular Biosciences » People » Daniel B Kim-Shapiro, Ph.D.

Daniel B Kim-Shapiro, Ph.D.

Daniel B Kim-Shapiro, Ph.D.
  • E-mail:
  • Phone Number: (336) 758-4993
  • Department: Physics

Study the effects of Nitric Oxide bioavailability in health, disease and therapeutics.  We study how the important signaling molecule nitric oxide is compromised in various disease states like sickle cell disease, transfusion of old stored blood, and various cardiovascular disorders.  We also work to help develop treatments that would restore nitric oxide bioavailability. 

Aggregation in biological systems, especially as it relates to diseases such as Sickle Cell Disease and other cardiovascular diseases.

Study how nitrite can be concerted to nitric oxide in normal physiology and in therapeutics.

Investigate the effects of nitric oxide and hydroxyurea on sickle cell hemoglobin using EPR and other spectroscopies. This work is conducted in collaboration with Bruce King of the Chemistry Department.

Study the mechanism and kinetics of sickle cell hemoglobin polymer melting (unaggregation).

Investigate the structure of the sickle cell hemoglobin fiber (aggregate).

 

 

Chenell Donadee, Nicolaas J.H. Raat , Tamir Kanias, Jesús Tejero,Janet S. Lee,  Eric E. Kelley, Xuejun Zhao, Chen Liu, Hannah Reynolds, Ivan Azarov , Sheila Frizzel, E Michael Meyer, Albert D. Donnenberg, Lirong Qu, Darrel Triulzi, Daniel B. Kim-Shapiro. and Mark T. Gladwin.   Nitric oxide scavenging by red cell microparticles and cell free hemoglobin as a mechanism for the red cell storage lesion.  Circulation.  124, 465-476, 2011

 

Mauro Tiso, Jesus Tejero, Swati Basu, Ivan Azarov, Xunde Wang, Virgil Simplaceanu, Sheila Frizzell, Thottala Jayaraman, Lisa Geary, Calli Shapiro, Chien Ho, Sruti Shiva, Daniel B. Kim-Shapiro and Mark T. Gladwin.  Human Neuroglobin functions as a redox regulated nitrite reductase.  J. Biol. Chem.  286, 18277-18289 2011. PMC3093900

 

Daniel B. Kim-Shapiro, Janet Lee, Mark T. Gladwin, Storage Lesion.  Role of Red Cell Breakdown.  Transfusion.  51, 844-851, 2011.  PMCID3080238

 

Mauro Tiso, Jesus Tejero, Swati Basu, Ivan Azarov, Xunde Wang, Virgil Simplaceanu, Sheila Frizzell, Thottala Jayaraman, Lisa Geary, Calli Shapiro, Chien Ho, Sruti Shiva, Daniel B. Kim-Shapiro and Mark T. Gladwin.  Human Neuroglobin functions as a redox regulated nitrite reductase.  J. Biol. Chem.  286, 18277-18289 2011. PMC3093900

 

 Tennille D. Presley, Andreas S. Perlegas, Lauren E. Bain, Samir K. Ballas, James S. Nichols, Hernan Sabio, Mark T. Gladwin, Gregory J. Kato, and Daniel B. Kim-Shapiro. Effects of a Single Sickling Event on the Mechanical Fragility of Sickle Trait Erythrocytes. Hemoglobin. 34, 24-36, 2010.

Swati Basu, Agnes Keszler, Natalia Azarova, Nneka Nwanze, Andreas Perlegas, Sruti Shiva, Katarzyna Broniowska, Neil Hogg, Daniel Kim-Shapiro. A Novel Role for Cytochrome c: Efficient Catalysis of S-Nitrosothiol Formation. Free Rad. Biol. Med. 48, 255-263, 2010.

Bradley I Goetz, Howard W Shields, Swati Basu, Pamela Wang, S. Bruce King, Neil Hogg, Mark T Gladwin, and Daniel B. Kim-Shapiro. An electron paramagnetic resonance study of the affinity of nitrite for methemoglobin. Nitric Oxide: Biology and Chemistry, 22, 149-154 2010.

Jon O Lundberg, Mark T Gladwin, Amrita Ahluwalia, Nigel Benjamin, Nathan S Bryan, Anthony Butler, Pedro Cabrales, Angela Fago, Martin Feelisch, Peter C Ford, Bruce A Freeman, Michael Frenneau, Joel Friedman, Malte Kelm, Christopher G Kevil, Daniel Kim-Shapiro, Andrey V Kozlov, Jack R Lancaster Jr, David Lefer, Kenneth McColl, Kenneth McCurry, Rakesh Patel, Joel Petersson, Tienush Rassaf, Valentin P Reutov, George B Richter-Addo, Alan Schechter, Sruti Shiva, Koichiro Tsuchiya, Ernst E van Faassen, Andrew J Webb, Brian S Zuckerbraun, Jay L Zweier and Eddie Weitzberg. Meeting Report: Nitrate and nitrite in physiology, nutrition and therapeutics. Nat. Chem. Biol. 5, 865-869, 2009.

Daniel Kim-Shapiro. Structure and function of hemoglobin and its dysfunction in sickle cell disease in Disorders of Hemoglobin, Genetics, Pathophysiology, and Clinical Management, 2nd Edition, (Martin H. Steinberg, Bernard G. Forget, Douglas R. Higgs, David J. Weatherall, editors), Cambridge University Press, 2009

Ernst E. van Faassen , Soheyl Bahrami, Martin Feelisch, Neil Hogg, Malte Kelm, Daniel B. Kim-Shapiro, Andrey V. Kozlov, Haitao Li, Jon O. Lundberg, Ron Mason, Hans Nohl , Tienush Rassaf, Alexandre Samouilov, Anny Slama-Schwok, Sruti Shiva, Anatoly F. Vanin, Eddie Weitzberg, Jay Zweier, Mark T. Gladwin. Nitrite as regulator of hypoxic signaling in mammalian physiology. Med. Res. Rev. 29, 683-741, 2009

Arlin B. Blood, Mauro Tiso, Shilpa T. Verma, Jennifer Lo, Mahesh S. Joshi, Ivan Azarov, Lawrence D. Longo, Mark T. Gladwin, Daniel B. Kim-Shapiro, and Gordon G. Power. Increased nitrite reductase activity of fetal versus adult ovine hemoglobin. Amer. J. Physiol. 292, H524-H533, 2009.

David I. Shapiro-Ilan, James F. Campbell, Edwin E. Lewis, Daniel B. Kim-Shapiro. Directional movement of parasitic nematodes in response to electrical current. J. Invertebr. Path. 100, 134-137 2009.

Swati Basu, Natalia A. Azarova, Michael D. Font, S. Bruce King, Neil Hogg, Mark T. Gladwin, Sruti Shiva, and Daniel B. Kim-Shapiro. Nitrite Reductase Actvity of Cytochrome c. J. Biol. Chem. 283, 32590.32597, 2008.

Mark T. Gladwin and Daniel B. Kim-Shapiro. The functional nitrite reductase activity of the heme-globins. Blood, 112, 2636-2647, 2008.

Ivan Azarov, Xiaojun He, Anne Jeffers, Swati Basu, Burak Ucer; Roy R Hantgan, Andrew Levy, Daniel B. Kim-Shapiro. Rate of Nitric Oxide Scavenging by hemoglobin bound to haptoglobin. Nitric Oxide: Biology and Chemistry. 18, 296-302, 2008.

Xiaojun He, Ivan Azarov, Anne Jeffers, Tennille Presley, Jodi Richardson, S. Bruce King, Mark Gladwin, and Daniel Kim-Shapiro. The potential of Angeli’s salt to decrease nitric oxide scavenging by plasma hemoglobin. Free Rad. Biol Med., 44, 1420-1432, 2008.

Rozalina Grubina, Swati Basu, Mauro Tiso, Daniel B. Kim-Shapiro, and Mark T. Gladwin. Nitrite reductase activity of hemoglobin S (sickle) provides insight into contributions of heme redox potential versus ligand affinity. J. Biol. Chem., 283, 3628-3638, 2008.

Swati Basu, Rozalina Grubina, Jinming Huang, Jeanet Conradie, Zhi Huang, Anne Jeffers, Alice Jiang, Xiaojun He, Ivan Azarov, Ryan Seibert, Atul Mehta1, Rakesh Patel, S. Bruce King, Neil Hogg, Abhik Ghosh, Mark T. Gladwin, Daniel B. Kim-Shapiro. Catalytic generation of N2O3 by a concerted nitrite reductase and anhydrase activity of hemoglobin. Nat. Chem Biol., 3, 785-794, 2007

Rozalina Grubina, Zhi Huang, Sruti Shiva, Mahesh S. Joshi, Ivan Azarov, Swati Basu,4, Lorna A. Ringwood, Alice Jiang, Neil Hogg, Daniel B. Kim-Shapiro, and Mark T. Gladwin. Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxy-hemoglobin. J. Biol. Chem., 282, 12916-12927, 2007

Kris T. Huang, Zhi Huang, Daniel B. Kim-Shapiro. Nitric Oxide Red Blood Cell Membrane Permeability at high and low Oxygen Tension. Nitric Oxide: Biology and Chemistry, 16, 209-216, 2007

Anne Jeffers, Mark T. Gladwin, and Daniel B. Kim-Shapiro. Computation of Plasma Hemoglobin Nitric Oxide Scavenging in Hemolytic Anemias. Free Rad. Biol. Med. 41, 1557-1565, 2006.

Mark T. Gladwin, Harold Raat, Sruti Shiva, Cameron Dezfulian, Neil Hogg, Daniel B. Kim-Shapiro, and Rakesh P. Patel. Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection and vasodilation, Amer. J. Physiol: Heart and Circ. Physiol. 291, 2026-2035, 2006.

Swati Basu, Jared D Hill, Howard Shields, Jinming Huang, S. Bruce King, Ph.D., Daniel B. Kim-Shapiro. Hemoglobin effects in the Saville Assay. Nitric Oxide: Biology and Chemistry. 15, 1-4, 2006.

Jinming Huang, Mamudu Yakubu, Daniel B Kim-Shapiro, and S. Bruce King. Rat Liver Mediated Metabolism of Hydroxyurea to Nitric Oxide. Free Rad. Biol. Med. 40, 1675-1681, 2006.

Daniel B. Kim-Shapiro, Alan N. Schechter, and Mark T. Gladwin. Unraveling the reactions of nitric oxide, nitrite and hemoglobin in physiology and therapeutics. Arteriosclerosis, Thrombosis, and Vascular Biol. 26, 697-705, 2006.

Kris T. Huang, Ivan Azarov, Swati Basu, Jinming Huang, Daniel B. Kim-Shapiro. Lack of Allosterically Controlled Intramolecular Transfer of Nitric Oxide from the Heme to Cysteine in the Beta Subunit of Hemoglobin. Blood 107, 2602-2604, 2006

Mark T Gladwin, Alan N Schechter, Daniel B Kim-Shapiro, Rakesh Patel, Neil Hogg, Sruti Shiva, Richard O Cannon, Malte Kelm, David Wink, Michael Graham Espey, Edward Oldfield, Ryszard M Pluta, Bruce Freeman, Jack Lancaster, Martin Feelisch & Jon O Lundberg,. The emerging biology of the nitrite anion. Nature Chemical Biology, 1, 308-314, 2005.

Ivan Azarov, Kris T. Huang, Swati Basu, Mark T. Gladwin, Neil Hogg, and Daniel B. Kim-Shapiro, Nitric Oxide Scavenging by Red Blood Cells as a Function of Hematocrit and Oxygenation. J. Biol. Chem, 280, 39024-39032, 2005.

Kris T. Huang, Agnes Keszler, Neil Patel, Rakesh P. Patel, Mark T. Gladwin, Daniel B. Kim-Shapiro, Neil Hogg, The reaction between Nitrite and Deoxyhemoglobin: Reassessment of the reaction kinetics and stochiometry, J. Biol. Chem., 280, 31126-31131, 2005.

Zhi Huang, Sruti Shiva, Daniel B. Kim-Shapiro, Rakesh P. Patel, Lorna A. Ringwood, Cythia E. Irby, Kris T. Huang, Chien Ho, Alan N. Schechter, Neil Hogg, and Mark T. Gladwin, Enzymatic nitrite reductase activity of hemoglobin generates NO under allosteric control, J. Clin. Invest., 115, 2099-2107, 2005

Anne Jeffers, Xiuli Xu, Kris Huang, Man Cho, Neil Hogg, Rakesh P. Patel, and Daniel B. Kim-Shapiro, Hemoglobin mediated nitrite activation of soluble guanylyl cyclase, Comp. Biochem. Physiol. Part A, 142, 130-135, 2005.

Fouad Azizi, Jerrold E. Kielbasa, Adedoyin M. Adeyiga, Rachel D. Maree, Matthew Frazier, Mamudu Yakubu, Howard Shields, S. Bruce King, and Daniel B. Kim-Shapiro, Rates of Nitric Oxide Dissociation from Hemoglobin, Free rad. Biol. Med., 39, 145-151, 2005.

Daniel B. Kim-Shapiro, Mark T. Gladwin, Rakesh P. Patel, and Neil Hogg, The reaction between nitrite and hemoglobin: The role of nitrite in hemoglobin-mediated hypoxic vasodilation, J. Inorg. Biochem. 99, 237-246, 2005.

Kejing Chen, Samir K. Ballas, Roy R. Hantgan, and Daniel B. Kim-Shapiro, Aggregation of Normal and Sickle Hemoglobin in High Concentration Phosphate Buffer. Biophys. J. 84, 4113-4121, 2004.

Daniel B. Kim-Shapiro, Rakesh P. Patel, Alan N. Schechter, Mark T. Gladwin, Richard O. Cannon, Neil Hogg, How do Red Blood Cells Dilate Blood Vessels?Reply. Circ. Res. 95, e10, 2004.

Christian J. Hunter, Andre Dejam, Arlin B. Blood, Howard Shields, Daniel B. Kim-Shapiro, Roberto F. Machado, Selamawit Tarekegn, Neda Mulla, Andrew O. Hopper, Alan N. Schechter, Gordon G. Power, and Mark T. Gladwin. Inhaled nebulized nitrite is a hypoxia-sensitive NO-dependent selective pulmonary vasodilator. Nat. Med., 10, 1122-1127, 2004.

Virginia L Lockamy, Howard Shields, Daniel B Kim-Shapiro and S. Bruce King. Iron Nitrosyl Hemoglobin Formation from the Reaction of Hydroxylamine and Hemoglobin under Physiological Conditions. Biochim. Biophys. Acta, 1674, 260-267, 2004.

Jinming Huang, Daniel B. Kim-Shapiro, S. Bruce King. Catalase-Mediated Nitric Oxide Formation from Hydroxyurea. J. Med. Chem., 47, 3495-3501, 2004.

Daniel Kim-Shapiro, Hemoglobin-nitric oxide cooperativity: Is NO the third respiratory ligand? Free Rad. Biol. Med. 36, 402-412, 2004

Kenyatta Cosby, Kristine S. Partovi, Jack H. Crawford, Rakesh P. Patel, Christopher D.Reiter, Sabrina Martyr, Benjamin K. Yang, Myron A. Waclawiw, Gloria Zalos, Xiuli Xu, Kris T. Huang, Howard Shields, Daniel B. Kim-Shapiro, Alan N. Schechter, Richard O. Cannon III, and Mark T. Gladwin. Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation. Nat. Med. 9, 1498 1505, 2003.

Xiuli Xu, Man Cho, Netanya Y. Spencer, Neil Patel, Zhi Huang, Howard Shields, S. Bruce King, Mark T. Gladwin, Neil Hogg, and Daniel B. Kim-Shapiro, Measurements of nitric oxide on the heme iron and b -93 thiol of human hemoglobin during cycles of oxygenation and deoxygenation. Proc. Natl. Acad. Sci. USA, 100, 11303-11308 2003.

Zhi, Huang, Leigh Hearne, Cynthia E. Irby, S. Bruce King, Samir K. Ballas, and Daniel B. Kim-Shapiro, Kinetics of Increased Deformability of Deoxygenated Sickle Cells Upon Oxygenation, Biophys. J., 85, 2003.

Virginia L. Lockamy, Jinming Huang, Howard Shields, Samir K. Ballas, S. Bruce King, and Daniel B. Kim-Shapiro, Urease enhances the formation of iron nitrosyl hemogolobin in the presence of Hydroxyurea, Biochim. Biophys. Acta, 1622, 109-116, 2003.

Xiuli Xu, Virginia L. Lockamy, Kejing Chen1, Zhi Huang, Howard Shields, S. Bruce King, Samir K. Ballas, James S. Nichols, Mark T. Gladwin, Constance T. Noguchi, Alan N. Schechter, and Daniel B. Kim-Shapiro, Effects of Iron Nitrosylation on Sickle Cell Hemoglobin Solubility, J. Biol. Chem. 277, 36787-36792, 2002.

Zhi Huang, K. B. Ucer, Timothy Murphy, , Richard T. Williams, S. Bruce King and Daniel B. Kim-Shapiro, Kinetics of Nitric Oxide Binding to R-state Hemoglobin, Biophys. Biochem. Res. Comm., 292, 812-818, 2002.

J. Huang, S. B. Hadimani, J. W. Rupon, S. K. Ballas, D. B. Kim-Shapiro and S. B. King. Iron Nitrosyl Hemoglobin formation from the reactions of Hemoglobin and Hydroxyurea, Biochemsitry 41, 2466-2474, 2002.

Z. Huang, J. G. Louderback,, F. Azizi, M. Goyal, S. Bruce King, and D. B. Kim-Shapiro. Nitric Oxide Binding to Hemoglobin in Oxygenated Blood. Biophy. Biochem. Acta. 1568 (3), 252-260, 2001.

Z. Huang, J. G. Louderback, S. B. King, S. K. Ballas and Daniel B. Kim-Shapiro. Hydroxyurea Reduces the Deformability of Sickle Red Blood Cells in Vitro. Am. J. Hematology 67, 151-156, 2001.

S. Kh. Aroutiounian, J.G. Louderback, S.K. Ballas, and D.B. Kim-Shapiro. Evidence for carbon monoxide binding to sickle cell polymers during melting. Biophys. Chem. 91, 167-181, 2001.

J. W. Rupon, S. R. Domingo, S. V. Smith, B. K. Gummadi, H. Shields, S. K. Ballas, S. B.King, and D. B. Kim-Shapiro. The Reactions of Myoglobin, Normal Adult Hemoglobin, Sickle Cell Hemoglobin and Hemin with Hydroxyurea. Biophys. Chem. 84 1-11 2000

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1.      Chenell Donadee, Nicolaas J.H. Raat , Tamir Kanias, Jesús Tejero,Janet S. Lee,  Eric E. Kelley, Xuejun Zhao, Chen Liu, Hannah Reynolds, Ivan Azarov , Sheila Frizzel, E Michael Meyer, Albert D. Donnenberg, Lirong Qu, Darrel Triulzi, Daniel B. Kim-Shapiro. and Mark T. Gladwin.   Nitric oxide scavenging by red cell microparticles and cell free hemoglobin as a mechanism for the red cell storage lesion.  Circulation.  124, 465-476, 2011.

 

 

Main Goal: My research focuses on understanding how blood flow is regulated, particularly by nitric oxide, nitrite and other nitrogen oxides. I rely on various forms of spectroscopy so I use light (including polarized light) to learn about biological structure and function.

Nitrogen oxide signaling in hemoglobin and other heme proteins in normal physiology, disease and therapeutics using various spectroscopies including EPR, light scattering, and time-resolved absorption.