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Molecular and Cellular Biosciences at Wake Forest University

Wake Forest University Graduate School » Molecular and Cellular Biosciences

Daniel B Kim-Shapiro, Ph.D.

Daniel B Kim-Shapiro, Ph.D.
Study the effects of Nitric Oxide bioavailability in health, disease and therapeutics.  We study how the important signaling molecule nitric oxide is compromised in various disease states like sickle cell disease, transfusion of old stored blood, and various cardiovascular disorders.  We also work to help develop treatments that would restore nitric oxide bioavailability. Aggregation in biological systems, especially as it relates to diseases such as Sickle Cell Disease and other cardiovascular diseases. Study how nitrite can be concerted to nitric oxide in normal physiology and in therapeutics. Investigate the effects of nitric oxide and hydroxyurea on sickle cell hemoglobin using EPR and other spectroscopies. This work is conducted in collaboration with Bruce King of the Chemistry Department. Study the mechanism and kinetics of sickle cell hemoglobin polymer melting (unaggregation). Investigate the structure of the sickle cell hemoglobin fiber (aggregate). Dr. Kim-Shapiro's Homepage


Main Goal: My research focuses on understanding how blood flow is regulated, particularly by nitric oxide, nitrite and other nitrogen oxides. I rely on various forms of spectroscopy so I use light (including polarized light) to learn about biological structure and function.

Nitrogen oxide signaling in hemoglobin and other heme proteins in normal physiology, disease and therapeutics using various spectroscopies including EPR, light scattering, and time-resolved absorption.